Lee k, lim jb, yum jh, yong d, chong y, kim jm, bla vim2 cassettecontaining novel integrons in metallo. Pseudomonas aeruginosa with acquired metallo beta lactamase determinants in a european hospital. Metallo beta lactamase producing pseudomonas aeruginosa in a healthcare setting in alexandria, egypt. Although mbls were first detected in spain in 2002, very few mbl producing p. Metallo beta lactamase producing pseudomonas aeruginosa and acinetobacter baumannii s john, balagurunathan r abstract this study aims in identifying mbls particularly zn requiring molecular class b enzymes produced by pseudomonas aeruginosa and acinetobacter baumannii. The mbls production should be studied when the strain showed a pro.
Metallobetalactamase producing pseudomonas aeruginosa in a. On november 1, 2016, a point prevalence survey conducted at a chicago skilled nursing facility with ventilated residents vsnf a to understand the prevalence of carbapenemaseproducing organisms. Evaluation of phenotypic tests for detection of metallo. Metallo beta lactamase in clinical pseudomonas aeruginosa isolate in a portuguese hospital and identi. Prevalence of metallolactamases producing pseudomonas. Molecular evolution of metallobetalactamaseproducing pseudomonas aeruginosa in a nosocomial setting of highlevel endemicity. Dec 20, 2004 pseudomonas aeruginosa is a leading cause of hospital. Detection of pseudomonas aeruginosa producing metallo beta lactamases in a large centralized laboratory. Metallobetalactamase producing pseudomonas aeruginosa.
Metallobetalactamase producer pseudomonas aeruginosa. Tem1 and tem2 hydrolyze penicillins and narrow spectrum cephalosporins, such as cephalothin or cefazolin. Concurrent determination of collagenase and biofilm formation. P409 metallobetalactamase in clinical pseudomonas aeruginosa isolate in a portuguese hospital and identi.
Multidrugresistant pseudomonas aeruginosa producing per1. Hopkins 1, daniele meunier 1, jacqueline findlay 1, nazim mustafa 1, helena parsons 2, rachel pike 1, laura wright 1, neil woodford 1. We report a study that developed an edta disk screen test and a molecular diagnostic assay for the detection of mblproducing pseudomonas aeruginosa. In this work we aimed at studying the occurrence of metallo beta lactamase mbl producing p. The aim of this study was to investigate the molecular epidemiological characteristics of metallo. Detection of metallobetalactamase producing pseudomonas. Verona integronencoded metallobetalactamaseproducing. To date, there is no gold standard test for its identification, which delays. Metallo beta lactamase producing pseudomonas aeruginosa. On november 1, 2016, a point prevalence survey was conducted at a chicago skilled nursing facility with ventilated residents to understand the prevalence of carbapenemase producing organisms in health care facilities in the chicago region.
Incidence of metallo beta lactamase producing pseudomonas. Delhi metallo beta lactamase 1 ndm1 producing pseudomonas aeruginosa. Carbapenemresistant isolates of pseudomonas aeruginosa producing metallo. A prospective study was performed over a period of 8 months in a tertiary care hospital. Pseudomonas aeruginosa, an important nosocomial pathogen is dif. Pseudomonas species from cattle dung producing extended. Prevalence of metallobetalactamaseproducing pseudomonas.
Droplet rather than aerosolmediated dispersion is the. Demise for antibacterial drug discovery has been created after the. Pseudomonas aeruginosa, epsilometer test, metallo beta lactamases, mbl encoding genes. Beta lactamase enzymatic activity can be detected using nitrocefin, a chromogenic cephalosporin substrate which changes color from yellow to red upon beta lactamase mediated hydrolysis. Four types of mbls, imp, vim, spm1, and gim1, have been reported in pseudomonas aeruginosa, which is a leading cause of nosocomial infections 3, 11, 29. In 2001, multidrugresistant pseudomonas aeruginosa carrying blavim mbl genes were found to be widespread approximately 20% of all p. It is one of the most difficult multidrug resistant microorganisms capable of producing metallo.
Moreover, a novel imptype mbl, bla imp65 was discovered and. One of the most important resistant mechanisms in gramnegative bacteria is extended spectrum. We report the case of a patient admitted to an intensive care unit, with sepsis caused by multidrugresistant sao paulo metallobetalactamase1producing p. Carbapenems, mainly imipenem and meropenem, are potent agents for the treatment of infections caused by multiresistant gram. So, the aim of this study was to determine the rates of biofilm production and metallo beta lactamase production mbl among the strains of pseudomonas aeruginosa. Among the isolates nonsusceptible to imipenem that were collected from 28 hospitals from 2000 to 2001, 44 11. An earlier study using green fluorescent protein gfpexpressing escherichia coli gfpe. Studies on nutritional requirements of pseudomonas aeruginosa for lipase production.
Combined disk cd method and double disk synergy test ddst were performed to detect metallo beta lactamase mbl producing p. Chapter 10 metallobetalactamase producer pseudomonas aeruginosa. This study aimed to characterize a comprehensive collection of mbl producing p. Though a notorious organism, its identification should not be limited to pigment production alone, as some.
From this it can be inferred that metallo beta lactamases producing pseudomonas aeruginosa are some what lower in incidence in our narayana general hospital. Endemic carbapenemresistant pseudomonas aeruginosa with. Harbourrelated genes on plasmids, increase the risk of resistance transmission among commonly reported hospital infections. Pseudomonas aeruginosa, an opportunistic pathogen causes community acquired infections and also hospital acquired infection. Extended spectrum and metalo betalactamase producing. Metallobetalactamase producing pseudomonas aeruginosa in. Detection and characterization of metallo beta lactamases producing pseudomonas aeruginosa a manoharan 1, s chatterjee 1, d mathai 1, sari study group 2 1 benjamin m pulimood laboratories for infection, immunity and inflammation bmpliii, department of medicine unit i and infectious diseases, christian medical college, vellore 632004, tamil nadu, india 2 surveillance of antimicrobial. Clinical and microbiological cure was obtained under appropriate antibiotic treatment. We conducted a casecontrol study in which 69 inpatients harboring bla imppositive pseudomonas aeruginosa and 247 control subjects with bla impnegative pathogens were investigated. Dissemination of carbapenemresistance among multidrug.
High incidence of multidrugresistant pseudomonas aeruginosa was isolated from dfu patients. An alarming rise in hospital outbreaks implicating handwashing sinks has led to widespread acknowledgment that sinks are a major reservoir of antibioticresistant pathogens in patient care areas. Health, general biological sciences beta lactamases chemical properties health aspects cancer patients medical examination diabetics pseudomonas aeruginosa infections diagnosis drug therapy. Metallo beta lactamase production is an important mechanism for carbapenem resistance of pseudomonas aeruginosawhich, represents an emerging public health challenge. Highlights metallo beta lactamases are important enzymes because they are involved in the breakdown of antibiotics action. Rapid communications recurrent pyelonephritis due to ndm1. Pseudomonas aeruginosa has emerged as a major healthcare associated pathogen that creates a serious public health disaster in both developing and developed countries. This study was carried out to determine the prevalence of metallo. Register your specific details and specific drugs of interest and we will match the information you provide to articles from our extensive database and email pdf copies to you promptly.
In pseudomonas aeruginosa, secondary beta lactamases with extended substrate specificity can be responsible for acquired resistance to the most powerful antipseudomonal beta lactams, such as expandedspectrum cephalosporins and carbapenems. Metallobetalactamase vim1, spm1, and imp1 genes among. First identification of pseudomonas aeruginosa isolates producing a kpctype carbapenemhydrolyzing betalactamase. Full text mechanisms of heteroresistance and resistance. Docquier j, luzzaro f, amicosante g, toniolo a, rossolini g. Journal of pathogens hindawi publishing corporation. So, the main aims of this study were to determine the rates of biofilm production and metallo beta lactamase production mbl among the strains of pseudomonas aeruginosa. The aim of this study was to determine the prevalence and pattern of antibiotic resistance, extended spectrum and metallo beta lactamase producing p. Biofilm and metallo betalactamase production among the.
During september 18november 19, 2018, cdc received 31 reports of vim. Although mbls were first detected in spain in 2002 3, very few mbl. Epidemiology and virulence of vim4 metallo beta lactamase producing pseudomonas aeruginosa isolated from burn patients in eastern algeria. Detection of pseudomonas aeruginosa producing metallo. Epidemiology and characteristics of metallo lactamase. Metallo beta lactamase, pseudomonas aeruginosa, multiresistance. Aims this study was carried out to detect mbl producing p. Emergence of metallo betalactamase producing pseudomonas aeruginosa in pakistan. A number of these enzymes have been described, including extendedspectrum serine beta lactamases esbls of groups 2be and. References centers for disease control and prevention. Oct 23, 2017 frequently encountered multidrugresistant bacterial isolates of p. During 20182019, cdc identified a multistate outbreak of a highly resistant strain of pseudomonas aeruginosa verona integronencoded metallo.
This study will necessitate the implementation of national antibiotic policy to be made and implemented in. Metallo beta lactamases are responsible for carbapenemsresistant pseudomonas aeruginosa. Goncalves universidade feevale, novo hamburgo, rio grande do sul, brazil msc at universidade federal do rio grande do sul, porto alegre, rio grande do sul, brazil abstract pseudomonas aeruginosa is an opportunistic gramnegative bacterium that is mostly involved with. The majority of patients were medical tourists who had weight loss surgery at a single facility in tijuana, mexico.
The carbapenems available for use in india are imipenem and meropenem. Phenotypic and genotypic methods for detection of metallo. Pseudomonas aeruginosa is a gramnegative rod unable to ferment glucose and is widely known as an opportunistic organism, frequently involved in infections of immunosuppressed patients, and also causes outbreaks of hospitalacquired infections 1. Nosocomial spread of pseudomonas aeruginosa producing the. Clinical and bacteriological characteristics of imptype. Kathiravan t, marykala j, sundaramanickam a, kumaresan s, balasubramanian t. These two enzymes are the most common plasmidmediated beta lactamases in gramnegative bacteria, including enterobacteriaceae, pseudomonas aeruginosa, haemophilus influenzae, and neisseria gonorrhoeae. Pseudomonas aeruginosa is an ubiquitous, gramnegative, rodshaped, monoflagellated bacterium, capable of causing serious infections in immunocompromised host. In march 2012, a patient with history of prior hospitalisation in serbia was diagnosed in france with acute pyelonephritis due to ndm1 producing p. Lactamase producing carbapenemresistant pseudomonas aeruginosa or vimcrpa.
Multidrugresistant pseudomonas aeruginosa producing per1 extendedspectrum serine. Increased mortality rates have been documented for patients infected with mbl producing pseudomonas aeruginosa, and these rates are especially due to. In future this scenario may change because the gene responsible for mbl production is carried on mobile genetic element resulting in rapid spread to sensitive bacteria in the hospital. There have been few clinical studies to define the optimal therapy for infections caused by esbl producing pseudomonas aeruginosa strains. Carlos juan, laura zamorano, ana mena, sebastian alberti, jose luis perez, antonio oliver, metallo. The bla vim2 gene was detected in 75% isolates and bla. Metallobetalactamase production is an important mechanism for carbapenem resistance of pseudomonas aeruginosawhich, represents an emerging public health challenge. Metallo beta lactamase producing pseudomonas aeruginosa and. We determined the occurrence of acquired metallo beta lactamase mbl producing bacteria in korean hospitals. The survey identified 20 patients with verona integronencoded metallobetalactamaseproducing carbapenemresistant pseudomonas aeruginosa. Epidemiology and virulence of vim4 metallobetalactamaseproducing pseudomonas aeruginosa isolated from burn patients in eastern algeria. A plate of muller hinton agar showing imipenem edta combined disc test indicating a m. Karadesai, prevalence of extendedspectrum beta lactamases, ampc beta lactamase, and metallo beta lactamase producing pseudomonas aeruginosa and acinetobacter baumannii in an intensive care unit in a tertiary care hospital, journal of. Pseudomonas aeruginosa has emerged as a major healthcare associated pathogen that creates a serious public health disaster in both develop ing and developed countries.
Pseudomonas aeruginosa strains that produce metallo beta lactamases mbls are becoming increasingly prevalent in wound infections. Pitout jd, gregson db, poirel l, mcclure ja, le p, church dl. Spectrum beta lactamase esbl producing resistant organisms, however after the emergence of new delhi metallo beta lactamase 1 ndm1 a recently evolved mbl has shown resistance to most classes of antibiotics including carbapenem except tigecycline, and colistin 10. Metallo beta lactamase producing pseudomonas aeruginosa and acinetobacter baumannii s john 1, r balagurunathan 2 1 department of biochemistry, indian institute of science, bangalore 560 012, india 2 department of microbiology, periyar university, salem 636 011, tamil nadu, india. Bloodstream infections with metallo lactamase producing pseudomonas aeruginosa. Phenotypic detection of metallo and ampc lactamases.
Phenotypic and genotypic methods for detection of metallo beta lactamase, volume 24 no. This study was designed to explore the dissemination of pseudomonas aeruginosa producing esbls on their plasmids recovered from the. Metallobetalactamases and resistance to carbapenems. Biofilm detection amongst metallo beta lactamase mbl. Resistance to lactams, including carbapenems, in pseudomonas aeruginosa may result from the acquisition of various lactamases, including metallolactamases mbls 1, 2. Finally, pcr was performed to detect mbl genes in mbl producing strains. The survey identified 20 patients with verona integronencoded metallo beta lactamase producing carbapenemresistant pseudomonas aeruginosa. Marra ar, pereira cap, gales ac, menezes lc, cal rgr, souza jma, et al. The aim of the present study is to determine the clinical features, incidence, and to find out the antimicrobial susceptibility pattern of pseudomonas aeruginosa in diabetic foot infections. Dec 31, 20 metallo beta lactamase mbl producing pseudomonas aeruginosa has emerged as a threat to hospital infection control, due to its multidrug resistance, especially in intensive care units icus. Metallo beta lactamase producing pseudomonas aeruginosa and acinetobacter baumannii.
376 6 338 1391 920 1447 1319 1010 409 832 76 1407 1488 1531 16 208 555 1262 1417 1398 415 494 217 1581 907 462 696 715 469 1273 566 234 583 749 868 617 839 906 279 602 1387 57 197